Abstract: The innovative outcomes can be achieved when personnel demonstrate organizational citizenship behavior (OCB) and through adequate structural empowerment measures that help achieving organizational goals and enhancing its effectiveness and productivity.Purpose: Assess organizational citizenship behavior among nurse managers and its relation to staff nurses' structural empowerment. Design: Descriptive correlational design was utilized in this study. Setting: The study was conducted at Menoufia University hospital including all 11 ICU units, all 7 OR units, all 18 inpatient departments and 1 outpatient clinics department. Subjects: Included all the nurse managers were 88 available at the time of the study, 377 staff nurses were selected by a systematic random sample to participate in the study. Tools: Organizational citizenship behavior and structural empowerment questionnaire. Results: The nurse managers' perception level regarding organizational citizenship behavior was high and the majority of staff nurses had a highly perception level regarding to structural empowerment and its dimensions. Conclusion: There was a significant positive relation between nurse managers' perception level regarding to organizational citizenship behavior and staff nurses' perception regarding to structural empowerment at Menoufia University hospital. Recommendations: Encourage nurse managers and staff nurses to practice and apply organizational citizenship behavior and structural empowerment as integral part of their daily work life through continuous workshops in the hospital.