Background. Total parenteral nutrition (TPN) is a medication used in the management and treatment of malnourishment. It is administration via central venous catheter for neonates who are unable to initiate normal nutrition. When nurses complained for applying infection control measures with this procedure that impact on preventing or decreasing central line associated blood stream infection, decreasing morbidity and mortality rate and improving medical service satisfaction. Aim. Evaluate nurses' application to infection control measures in TPN administration via neonatal jugular central venous catheter. Method. A cross-sectional research design was conducted in this study used purposive sampling technique to select the participants. All nurses(40)who are working in neonatal surgical intensive care unit of Mansoura University Children's Hospital. Tools. The researchers developed two tools for data collection. Tool 1 structured interview questionnaire which consist of two-parts; part 1nurses' socio-demographic and occupational characteristics, part 2 assessed nurses' knowledge regarding infection control measures related to TPN administration. Tool 2 an observation checklist to assess nurses 'application of infection control measures pre, during and post TPN.
Results. The majority of nurses had poor knowledge related to TPN administration and had unsatisfactory level of performance related to infection control measures pre, during and post TPN administration via neonatal jugular central venous catheter. Conclusion. There is no significant correlation between nurses' knowledge and their performance related TPN administration via neonatal jugular central venous catheter. Recommendations: conducting regular in-services, training programs to improve nurses' knowledge and performance regarding care of TPN administration via neonatal jugular.