Background: Hematopoietic stem cell transplantation is a complex and singular treatment used for hematological and
non-hematological diseases. Preparation related to hematopoietic stem cell transplantation reduce feeling of anxiety and
improve quality of life. Aim: Evaluate the effect of stem cell transplantation preparation on patients′ knowledge and
anxiety level. Methods: Quasi-experimental research design was used. Setting: The study was conducted at Oncology
center, Mansoura University, Egypt. Subject: A purposive sample of 27 patients of both sexes who were willing to
participate in the study. Tools: Two tools were used; Tool I Interview questionnaire sheet and Tool II State-Trait
Anxiety Inventory (STAI). Results There was a statistically significant improvement in patients′ knowledge level
regarding stem cell transplantation post- and follow up compared to before implementation of transplant preparation.
The level of anxiety among stem cell transplantation patients decreased post-transplant and follow (48.1%-40.7%
respectively) compared to pre implementation of transplant preparation (81.5%)Conclusion: Preparation of patients
undergoing hematopoietic stem cell transplantation had a great value in enhancing patients′ knowledge and reducing
anxiety level. Recommendations: Written instruction related to preparation about stem cell transplantation should be
available for all patients undergoing stem cell transplantation.