The coronavirus disease-19 pandemic (COVID-19) has challenged health systems worldwide, especially the health workforce, a pillar crucial for health system resilience. Therefore, this study aimed to assess healthcare workers' (HCWs) performances related to the COVID-19 pandemic in healthcare settings. This was a descriptive cross-sectional study designed on 339 HCWs in healthcare settings in Diarb Al-Souk village and Diarb Negm City, Sharkia. To gather the data, the researchers employed a structured self-administered questionnaire to identify socio-demographic and occupational characteristics, as well as a structured observational checklist to evaluate performance. The current study shows that 99.7%, 94.7%, and 61.9% of HCWs exhibit inadequate performance concerning the "five moments of hand hygiene," the "hand hygiene technique with soap and water," and with alcohol-hand-based rub, 67.8%, 100%, 71.7%, 95%, 99.4%, 100%, and 99.7% toward hand hygiene, wearing a gown, mask with ties, mask with elastic headbands, respirator, goggles or face shield, and gloves in sequence for downing personal protective equipment (PPE), respectively. Also, incompetent performance is observed in 100% of PPE as follows: gown, respirator removal by touch technique, respirator removal by non-touch technique, and eyewear or face shield removal. In addition, 90% and 97.6% of the HCWs incompetently performed the mask with ties and the removal of the gloves, respectively. The researchers conclude that incompetence is observed in HCWs' performance during the COVID-19 pandemic. Finally, the researchers recommend that scheduled on-the-job training programs be conducted for all HCWs on COVID-19 pandemic infective preventive measures, their performance, and close monitoring and supervision of HCWs to ensure that they strictly adhere to COVID-19 pandemic infection prevention guidelines.