Background: Silver oxide nanoparticles (AgNPs) have many uses as antiviral materials that cause diseases, they are considered AgNPs to destroy bacteria, they can also be considered as antidotes for many serious diseases such as cancer and viruses, on the creation and characterization of silver oxide nanoparticles (AgNPs) and investigate the impact of varying doses of AgNPs on the antioxidant defense system, as well as the histology and immunohistochemistry of male rats liver. Methods: There were fifty male rats (150–170 g) utilized. The animals were split up into five groups, each with ten rats treated for three weeks, the first group served as the control, while groups II, III, IV, and V received oral treatment with AgNPs at doses of 1/150 LD50, 1/100 LD50, 1/50 LD50, and 1/25 LD50 mg/kg BW/day. After the trial, liver and blood samples were taken to look at various factors. Results: Silver nanoparticles (AgNPs) were prepared at different concentrations, used in laboratory animals, and characterized using different instruments. treatment with silver nanoparticles (AgNPs) in different concentrations Changes in histopathology and immunohistochemistry were noted, confirming that biochemical disruptions were caused by silver nanoparticles (AgNPs) in distinct amounts in the liver of rats. Conclusion: It is evident that silver nanoparticles (AgNPs) Produced significant negative effects in rat liver tissue and p53 protein in a dose-dependent way.