Background: The cultural intelligence of head nurses plays a crucial role in the quality of their job performance, encompassing clinical decision-making, critical thinking, evidence-based practice, and knowledge application. Hence, the proficient nurse must possess the capacity to comprehend the perspectives and requirements of a diverse range of individuals in order to be driven, assisted, and content. The aim of the research: was to examine the effect of a head nurse's cultural intelligence training program on staff nurses' agility at work and career aspiration. Research design: quasi-experimental research design was utilized in this study. Setting: the study was conducted at the liver disease Hospital in Minia governorate. Subjects: a convenience sample of head nurses and staff nurses was included in the study. Data collection: the study utilized four instruments: the cultural intelligence knowledge questionnaire, the cultural intelligence self-assessment questionnaire, the agility scale, and the career aspiration scale. Results: prior to the program implementation, the head nurses had a low level of cultural Intelligence and its subscales. However, after the program implementation, their level significantly increased to a high level. Furthermore, the work agility and career aspirations of the staff nurse experienced a boost following the implementation of a cultural intelligence educational program specifically designed for head nurses. Conclusion: an improvement in the knowledge level has been observed after the program was implemented. Recommendation: periodical workshops and programs for head nurses to develop their cultural intelligence competencies to be more flexible, agile, and career aspirations.