Background: Toxoplasmosis is one of the most dangerous infections during pregnancy that is
associated with severe life-threatening complications. The aim of this study was to evaluate the
effect of the educational instructions on pregnant women's knowledge and attitudes regarding
toxoplasmosis. Subjects and methods: A quasi experimental study design was utilized. This study
was conducted at the antenatal units (inpatient and outpatient) of obstetric departments of Tanta
University hospitals and El-Menshawy hospital. A purposive sample of 100 pregnant women was
included in the study. Two tools were used; Tool I: Pregnant women's knowledge regarding
toxoplasmosis. Tool II: Pregnant women's attitudes regarding toxoplasmosis. Results: More than
four fifths (83.0%) of the studied pregnant women had low level of knowledge regarding
toxoplasmosis pre the educational instructions while most (93%) of them had high level of
knowledge immediately with slight decrease one month post implementation of the educational
instructions and nearly one fifth (18%) of them had positive attitude regarding toxoplasmosis pre the
educational instructions compared to most (90%) of them immediately which becomes (97%) one
month post the educational instructions. Conclusion: The studied pregnant women's knowledge
regarding toxoplasmosis had significantly improved immediately with slight decrease one month
post implementation of the educational instructions and they had positive attitudes regarding
toxoplasmosis immediately with obvious improvement one month post implementation of the
educational instructions. Recommendations: The mass media should be utilized to disseminate
correct and relevant information about toxoplasmosis to women, families, and the community.