Abstract Background: Premarital therapy often describes a procedure aimed to better premarital relations, resulting in stable marriages and greater satisfaction. Aims: To assess studied students' knowledge and attitude regarding premarital examination at Aswan University. Design: A descriptive cross sectional study. Setting: This study was conducted at four randomly selected faculties at Aswan University. Sample: A purposive sample of 310 of students at level fourth at Aswan University was recruited in this study. Tools: Two tools; Tool I: An self-administrated questionnaire sheet was involving two parts: part 1: Socio-demographics, part 2: Knowledge assessment questionnaire Tool II: Attitude Assessment Scale. Results: 47.1% of the students had poor knowledge level, while 22.6% of them had good knowledge level regarding pre-marital examination. Also, 61.3% of the students had positive attitude regarding pre-marital examination, while, 38.7% of them had negative attitude regarding premarital examination. Conclusion: there was significant correlation (P=0.028) between the total students' knowledge and total attitude regarding premarital examination Recommendations: Developing an educational program to raise awareness of students about premarital counseling in universities.