Background: Researchers, practitioners, and policy makers in the field of health care are increasingly focusing on evidence-based practice. It has gained power as a strategy that can affect nursing professionals' knowledge and practice. Aim of the study: To determine the association between evidence based practice sustainability and perceived nurses' obstacles at pediatric critical departments. The study's design: A descriptive design was used in this study. The sample is a convenience sample of 114 nurses affiliated at pediatric critically departments (NICU, PICU) in Assuit university children hospital. Tools: Three tools were utilized in the present study. Tool (1): Sociodemographic data of pediatric nurses & nurses' knowledge regarding EBP, Tool (2): Nurses' practice regarding EBP & nurses' attitude towards EBP and Tool (3): Perceived nurses' obstacles regarding EBP. Results: Approximately two-thirds (70.2%) of the studied nurses had satisfactory knowledge, practice, and attitudes relating EBP, and (64.9%) of nurses perceive obstacles to implement EBP. Conclusion: There is a considerable negative association between perceived obstacles and nurses' knowledge, practice, and attitude towards EBP. Recommendations: Courses emphasizing evidence-based practice were offered. Theoretical knowledge and practical skills should be included in these programs to guarantee that nurses stay current with developments in nursing practice based on evidence