Background: Workplace bullying is viewed as a very harmful issue in the nursing field, where bullying is frequently seen as a normal part of the work environment Aim: Investigate the effect of workplace bullying on nursing staff' work practice environment and satisfaction Subjects and Method: A descriptive correlational design was conducted at Assiut University Hospitals for simple random samples was used to choose a number of nurses & all head nurses and collect the study data by three tools: (1) include two parts, (I) Personal &job characteristics, (II) workplace bullying scale, (2) work practice environment of the nursing work index and (3) work satisfaction scale. Results: The highest mean scores of workplace bullying in oncology hospital, while, the highest mean scores of work practice environment in Al Rajhy hospital among the studied subject and higher than three quarter of the studied subject in women health hospital have a moderate level of work satisfaction (80%,). Conclusions: There were negative correlation between nursing staff' work practice environment and satisfaction with workplace bullying. While there was a positive correlation between nursing staff' work practice environment and satisfaction. Recommendations: Conducting training program for nurses about the strategies & techniques to dealing with workplace bullying.