Background: Vaginal yeast infection is uncomfortable for the mother and can pass to the fetus during delivery, leading to maternal and neonatal complications. Vaginitis is more common and the risk of recurrence is higher during pregnancy. Aim: To assess woman's knowledge of vaginal yeast infection among pregnant women. Methods: Descriptive cross sectional design was used in this study. Setting: This study conducted at the out patient's antenatal clinics of Woman's Health Hospital, Assuit University. Sample: A sample of 370 pregnant women was included in the study. Tool a structured interviewing questionnaire used to assess women's knowledge about yeast infection during pregnancy, part I consist from demographic, obstetrical and family planning data, part II to assess women's knowledge about yeast infection. Results: The current study findings illustrated that more than half (56.5%) of the studied women had moderate level of knowledge about vaginal yeast infection. Conclusion: More than two third of the studied pregnant women had moderate and poor level of knowledge regarding yeast infection. Recommendations: Guidance and health education are important for pregnant women, to improve their knowledge regarding yeast infection.