Background: Green hospital facility aims to improve patient wellbeing, help in the healing process, and make efficient use of natural resources without harming the environment. Patient safety is the avoidance and prevention of patients' injuries or adverse events resulting from the processes of health care delivery. Aim: To evaluate the effect of educational program on perception of nurses regarding green hospital and their attitude towards patient safety guidelines. Research design: A quasi experimental pre-post-test design was utilized to achieve the aim. Setting: The study was conducted at Nasser Institute, affiliated to the secretariat of specialized medical centers, Ministry of health, Cairo, Egypt. Sampling: A purposive sample of 107 nurses. Tools: (I) A) Structured sociodemographic and work data. B) Pre-post perception questionnaire regarding smart hospitals initiative green hospitals. (II), Pre-post attitude scale towards patient safety guidelines. Results: The total positive green hospital perception of nurses was improved from 6.5% to 90.7% after the intervention. Also the total positive attitude towards patient safety guidelines by nurses was improved from 8.4% in pre-test to 86.9% in post-test. In addition to a positive significant correlation between nurses' total green hospital perception and attitude towards patient safety guidelines. Conclusion: The educational program detected improvement in the positive nurses' perception regarding green hospital and their positive attitude towards patient safety guidelines, with a strong positive correlation. Recommendation: Green hospital and patient safety guidelines should be implemented nationally to be in line with the sustainable development goals.