Background: Pain relief in the course of labor is often neglected. Providing care and comfort to parturient women all through childbirth is a necessary role. Using non pharmacologic skills to support the ordinary system of birth, nurses may also minimize the use of medical methods and their viable complications. Aim: Assess the role of nurses in providing pain relief for women in labour. Research Question: Do nurses have a role in relieving pain to women in labour at Woman's Health Hospital, Assiut University? Design: A clinical audit was used. Setting: It was conducted at reception and emergency unit, Woman's Health Hospital, Assiut University. Sample: A simple random sample of sixty two maternity nurses who deal with female in labour. Tool: There had been three tools concerned in this study:A structured Interviewing questionnaire, observational checklist, likert mind-set scale. Results: about 37.1% and 82.2% of the studied nurses had an efficient knowledge about pain-relief methods in pre and post audit respectively, 22.6% and 77.4% had pleasant practices in pre and post audit respectively. Also 54.8% and 96.8% of the studied nurses had a tremendous mind-set regarding pain-relief strategies in pre and submit audit respectively with enormously large distinction between pre and post audit p-value <0.01. Conclusion: There was a highly significant improvement in nurses' knowledge and practices, also attitude was improved. Recommendations: Implementation of labor supportive measure guidelines in different child- birth units to improve nurses' practice regarding pain relief measures.