Background and aim: Venipuncture is scary aspect for hospitalized children. Virtual Reality (VR) is considered inventive distraction technique for controlling pain among children experiencing painful procedures. The aim of the study was to evaluate the effect of virtual reality on alleviating children's fear and pain during painful procedures. Design: One-group pre- posttest quasi experimental research design was utilized. Sample: A Purposive sample of 30 hospitalized children experiencing painful procedures were participated in the study. Data were collected within 6 month using socio demographic data tool that developed by the researchers, Wong-Baker faces pain rating scale (WBFPS) and children's fear scale (CFS). Setting: The study was carried out at Pediatric Inpatient Surgical Unit in Cairo University Specialized Pediatric Hospital (CUSPH) and Pediatric Inpatient Medicine Units in Elmonira Pediatric Hospital. Results: The majority of children felt severe pain and extreme fear during painful procedure preVR while most of children felt less pain and fear post-VR. There were a highly statistically significant difference between the total mean scores of pain and fear pre-VR and post-VR. Conclusion: Virtual reality has been to be effective in reduction of pain and fear during venipuncture among hospitalized children. Recommendations: It is suggested that VR could be integrated as adjunctive non-pharmacological therapy with the routine care during painful procedures for hospitalized children.