Background: Awareness of hospital accreditation standard and its application is prerequisite to successful organizational learning culture. Aim: Determine nurse's awareness regarding hospital accreditation standards and assess organizational learning culture. Design: Descriptive design Setting: The research was carried out at South Egypt Cancer Institute in Assiut City. Subject: Includes (200) nurse, Methods: Three data collection tools: Personal data sheet, Hospital accreditation standards awareness questionnaire and Organizational learning culture questionnaire. Results: More than one third of nurses aware of hospital accreditation standard dimensions, but less than two third of nurses were not aware about hospital accreditation standard dimensions. Less than half of nurses had agreed that their organization had high organizational learning culture, meanwhile more than half of nurses had agreed that their organization had low organizational learning culture. Conclusion There are positive correlations between both awareness regarding hospitals accreditation standards and organizational learning culture dimensions. Recommendations: Developing strategies to support continuous learning to promote a strong organizational learning culture , and development opportunities to healthcare provider on a regular basis, further researches to test application of hospital accreditation standard and its effect on suitable or sustainable development goals (SDG)