Background: Pro-innovative characteristics are crucial in promoting nurses' innovative behavior and enhancing their perceived work role performance. Encouraging these characteristics through training and development programs can help healthcare organizations improve their services and promote a culture of innovation Aim: To investigate the effect of pro-innovative organizational and individual characteristics on nurses' innovative behavior and perception of work role performance. Methods: This cross-sectional study was conducted at Suez Canal University Hospitals and included 355 staff nurses from surgical and medical departments, as well as intensive care units (ICUs). Data was collected using a self-administered questionnaire consisting of the Corporate Entrepreneurship Assessment Inventory tool, Creative Efficacy scale, Proactivity scale, Employee Innovation Behavior scale, and Work Role Performance Scale Results: 47.3% of studied nurses had high Organizational characteristics while 52.7% of them had low. Also, 80.6% of them had high Individual characteristics and 72.4% of them had high innovative behavior. In addition, this table detects that 81.1% of studied nurses had high perception of work role performance. Conclusion: There was high positive correlation between organizational characteristics, individual characteristics, innovative behavior with work role performance. Also, there was high positive correlation between organizational and individual characteristics with the innovative behavior. Recommendations: Organizations should encourage and foster innovative behavior among nurses. This can be done through various means such as providing training opportunities, establishing innovation teams, and offering incentives for innovative ideas. Organizations should provide support for nurses in maintaining a work-life balance, especially for those who are married or have other caregiving responsibilities.