This study investigated the effect of mouth rinsing of carbohydrate solution on physical performance and some physiological variables during a workout. Seven (n = 7) collegiate athletes participated in the study. The physiological characteristics, in mean and standard deviation (M ± SD) of the sample, were Age (25.6 ± 4.3 y), weight (79.6 ± 22.7 kg), Height (172.3 ± 6.7 cm), BMI (26.6 ± 6.4 kg/m2), Percent of Body Fat (17.7 ± 6.9 %), and VO2 Max (49.5 ± 6.8 ml/min.kg-1). All subjects completed four experimental trials; the first visit was to collect baseline data and the subjects' physical characteristics. The three trials afterward were done in a crossover design. These trials consisted of a control test. Carbohydrate Mouth Rinsing (CMR) test: The subjects rinsed their mouths in (25 ml) of CHO solution for (10 seconds) every (15 minutes). The third test was a placebo test, mouth rinsing in (25 ml) of colored water for (10 seconds). They ran on a treadmill to exhaustion throughout the three trials. There was a significant difference in the Maximum Heart Rate (Max HR) and Body Temperature at (P ≤ 0.05). No significant differences were found in the physiological variables for the other variables. The use of CMR should be carefully considered as a protocol for enhancing sports performance. Appropriate dietary and fluid strategies should be prioritized during both training and competition. The study emphasizes the need for further research in this area, highlighting its importance and potential impact on sports performance enhancement.