In global, microorganisms-mediated water pollution is one of the major concerns for the aquatic environment. The fecal matter waste from hospitals, industry, and cattle farms increases the bacterial load in a water system. Hence, the main objective of this work is to investigate the validity of water crop irrigation that may be polluted with bacterial microbes and represents a source of microbiological health risk if not treated. In the present study water samples were collected from 1^st and 6^th districts in N'Djamena city according to the procedure recommended by American Public Health Association (APHA). The results were evaluated in logarithm as colony forming units per milligram (CFUs/mg) and also, were quoted at P < 0.05 levels of significance by using the statistical analysis program Rrecomander (R×643.2.5. Lnk), then, were compared with the values ​​obtained from The World Health Organization (WHO) in Nigeria and other health, environmental institutions. Consequently, it was found that the microbial load of all collected irrigation water samples was estimated as 7.02×10², 9.85×10² and 3.89×10² log cfu/mg for total aerobic mesophilic flora (FAMT), total fecal bacteria and for Staphylococcus aureus, respectively. The observed counts in water irrigation samples were higher than the recommended standards of 1x10³ CFUs/mg. This experiment was performed in Bacteriology Laboratory of the livestock Research Institute for development of the Ministry of livestock and Animal Production.