The Background: Cancer treatment is one of the global challenges in the world. The type and amount of certain dietary components such as sugar could affect response to chemotherapeutic treatment. This research was performed to detect the influence of table sugar, brown sugar and sugar substitute (xylitol) with three different concentarations on the chemosensitivity of squamous cell carcinoma treated with cisplatin chemotherapy. Material &Methods: Dulbecco's modified Eagle medium supplemented with 10% foetal bovine serum was used to cultivate the squamous carcinoma cell line. Cisplatin chemotherapy was added according to the manufactory's guide to culture media. The squamous cell carcinoma cell line was subjected to table sugar, brown sugar, and sugar substitute (xylitol) at 10%, 30%, and 75% concentrations. Cytotoxicity of the cancer cells was detected by MTT assay, in addition cell death (apoptosis) was measured by flow cytometry. Results: Statistical analysis of the collected data revealed that increasing the amount of any type of the three kinds of sugar lead to a significant decrease of chemosensitivity of squamous cell carcinoma (P-value<0.0001). Moreover, the table sugar resulted in a significant increase in chemoresistance of cancer cells concerning cell viability and cell death when compared with brown sugar and sugar substitute(p-value≤0.05).Conclusion: It has been concluded that the worst type of sugar is table sugar followed by brown sugar that increases cancer cell resistance during chemotherapy treatment. However, xylitol (sugar substitute) could be considered as the best type of sugar that can be used without marked affection on the chemosensitivity of cancer cell.