Background: Alport syndrome is a hereditary kidney disease that gradually deteriorates kidney function and frequently leads to renal failure. A multitude of detrimental effects, such as mothers becoming more concerned and anxious about their children's physical and mental health, can result from certain forms of Alport syndrome, which also create various symptoms and indicators in children, such as hearing loss and eye abnormalities. Aim: To evaluate the effect of social-platform instructional guidelines on pediatric mothers' knowledge, and anxiety regarding Alport Syndrome. Design: to fulfill this study a quasi-experimental research design was used. Setting: This study was conducted in Sohag City, Egypt. Sample: A total purposive sample of 300 mothers from 10 and 30 July 2023 was obtained using an online questionnaire via Google Forms. Tools: Three tools were utilized: Tool I: Nurses' knowledge regarding Alport Syndrome, Tool II: Future anxiety scale, and Tool III: Nurses' satisfaction with social-platform instructional guidelines implementation. Results: The study showed that nurses' understanding of Alport Syndrome was higher after the social platform instructional guidelines were implemented than before. After the social-platform instructional instructions were implemented, the moms in the study experienced a reduction in their anxiety levels. Mothers' overall awareness of Alport Syndrome and their anxiety levels were positively correlated, with a significance level of 0.01 after implementation. Conclusion: The study concluded that mothers' knowledge and anxiety levels are improved when social media platforms are used to implement instructional guidelines about Alport Syndrome. Recommendations: A well-thought-out health education program that enhances mothers' understanding of Alport Syndrome and reinforces potential interventions is crucial for supporting and assisting with psychological well-being.