Background: Urinary tract infection is the most common healthcare associated infection that usually results from prolonged use of urinary catheter and results in prolonged hospital stay. Prevention of catheter associated urinary tract infection in pediatric patients regarded now as an important hospital policy worldwide. Aim of the study: This study aimed to assess nurses' role to prevent urinary tract infections for neonate with urinary catheter. Design: A descriptive research design was utilized to achieve the aim of this study. Setting: The study was conducted at two neonate's intensive care units; Maternity and Gynecological Hospital affiliated to Ain Shams University Hospitals and Benha Specialized Pediatric Hospital affiliated to Ministry of Health. Study subjects: A convenient sample of 40 nurses who represent all available nurses in the previous mentioned setting. Data collection tools: A Pre-Designed Questionnaire Sheet and Catheter associated urinary tract infection bundle. Results: The study showed that, the mean age of the studied nurses was 24.29±4.19 years, 85.0 % of nurses were female, 40.0% of them had institute level of education and 45.0% of them had <5 years of experience. Regarding level of knowledge, 70.0% of studied nurses had poor knowledge prevention of urinary tract infection for neonate with urinary catheter and 90.0% of nurses had incompetent practice regarding urinary tract infection prevention for neonate with urinary catheter. Conclusion: The study findings concluded that, more than two thirds of studied nurses had poor level of knowledge and most of them had incompetent level of practice about urinary tract infections for neonates with urinary catheter. Also, a significant statistical positive correlation was found between knowledge of the studied nurses and their practice. Recommendation: A similar study should be replicated on a large sample and other place to generalize the findings.