Background: Arterial blood gas analysis is a vital diagnostic tool used in the medical field to assess a patient's respiratory and metabolic status Aim of the study: The study aimed to assess the effect of educational guidelines on nurses' performance regarding arterial blood gases sampling and interpretation Design: A Quasi experimental research (pre/post test) design was utilized in this study. .Setting: The study was carried out at the Intensive Care Units of Emergency Hospital at Mansoura University. Subjects: A convenient sample of all available nurses (50) working at the Intensive Care Units of Emergency Hospital at Mansoura University Tools: tool I Nurses Self-administered questionnaire consists of two parts part 1: Demographic characteristics of nurses, part 2: Nurses' knowledge Assessment Questionnaire. Tool II: Nurses' practice Observational checklist for ABG sampling. Results: More than two third, 70.0 % of nurses had satisfactory level of total knowledge regarding arterial blood gases sampling and interpretation post - educational guidelines implementation. Also, the majority, 96.0% of them had satisfactory level of total practice regarding arterial blood gases sampling and interpretation post educational guidelines implementation. Conclusion: Based on the results of the present study it can be concluded that  the educational guidelines were found to be  highly effective method to improve the nurses' knowledge and practice related arterial blood gases sampling and interpretation guidelines. Recommendations: Based on the current study finding: the following recommendation was proposed:  Create online platforms where healthcare professionals can share their experiences, knowledge, and practices regarding arterial blood gases sampling and interpretation.