Background: There is a vast growth of Artificial Intelligence (AI) applications across all aspects of healthcare. Nursing practice is critical and AI technology will enhance practice and patient outcomes. The study aimed to investigate the effect of educational guidelines on maternity nurses' knowledge and attitude regarding Artificial Intelligence applications. Design: A quasi-experimental research design with a pre/post-test was utilized. Setting: This study was conducted at Sohag University Hospital's obstetrics department. Sample:- A  convenience sampling of all nurses working in the previously mentioned setting, Egypt. Tools: Tool (1): A self-administered Nurses' Knowledge Questionnaire regarding Artificial Intelligence and Tool (2): Nurses' General Attitudes towards Artificial Intelligence Questionnaire. Results: Pre-implementation of the educational guidelines, 90% had unsatisfactory total knowledge scores and 88% had a negative attitude. After implementing the instructional guidelines, 92% had satisfactory total knowledge scores, and 96% had a positive attitude with statistically significant differences. Moreover, highly statistically significant differences between the demographic characteristics and total knowledge level post-implementation of the educational guidelines at (P<0.00). Conclusion: The educational guidelines that were conducted in this area had a significant effect on the promotion of maternity nurses' knowledge and attitude regarding Artificial Intelligence applications. Recommendations: maternity nurses should be provided with in-service training programs related to Artificial Intelligence applications and well-informed continuous; educational guidelines should be imparted to them.