Background: Artificial intelligence applications have grown vastly across all aspects of healthcare.
Nursing practice is critical and AI technology will enhance practice and patient outcomes. The
current study aimed to determine the impact of instructional guidelines on surgical nurses'
knowledge and attitude regarding artificial intelligence applications. Design: A quasi-experimental
design was utilized to fulfill the aim of this study. Setting: This study was conducted in surgical
departments at Sohag University Hospitals. Sample: A convenience sample included (50) nurses
were selected from the previously mentioned settings. Tools: Two tools were used to collect the
data: A self-administered Artificial Intelligence Knowledge Questionnaire and A General Attitudes
towards Artificial Intelligence Questionnaire. Results: The total level of knowledge was satisfactory
among 12% of studied nurses during the instructional guidelines period, while it was 88% post
instructional guidelines. Additionally, the total positive nurses' attitudes mean score improved from
44.26 ±22.08 pre-instructional guidelines to 81.07±17.54 post-instructional guidelines with
statistically significant differences. Moreover, highly statistically significant differences between
total knowledge and attitude level post-intervention (P<0.001). Conclusion: This study concluded
that the instructional guidelines had a significant positive effect on improving the studied nurses'
knowledge and attitudes regarding artificial intelligence applications. Recommendation: encourage
nurses to increase their knowledge and attitudes toward artificial intelligence through attendance
workshops and training programs regarding artificial intelligence applications, which are required to
enable them to integrate artificial intelligence applications into nursing practices.