Background: Mothers of children with Burkitt lymphoma play a crucial role in the process of children's management and fulfilling the children's needs during the treatment period. Aim: To evaluate the effectiveness of instructional guidelines on anxiety and coping strategies among mothers having children with Burkitt's Lymphoma. Research Design: A quasi-experimental design with one group undergoing pre- and post-testing to achieve the aim of this study was used. Subjects: The purposive sample of 50 mothers of children with Burkitt‟s lymphoma was recruited. Setting: This study was carried out in the out- patients clinic in the pediatric oncology center at Mansoura university. Tools of data collection: Tool I: Structured questionnaire sheet, Tool II: Mother's knowledge regarding Burkitt's Lymphoma, Tool III: The mother's reported practice regarding the health needs and problems of their children with Burkitt's Lymphoma, Tool IV: (F-COPES) questionnaire and Tool V: Hamilton anxiety scale. Results: Findings revealed that there was a highly statistically significant reduction in anxiety among mothers and their children with Burkitt's lymphoma after instructional guidelines about coping strategies implementation than before implementation. Conclusion: The result was concluded that implementation of the instructional guidelines about coping strategies had a positive effect on reducing anxiety among mothers and their children with Burkitt's lymphoma after implementation compared to before. Recommendations: Generalization of instructional guidelines about coping strategies for all mothers and their children with Burkitt's lymphoma to alleviate their anxiety is recommended. Setting up emotional support groups to evaluate mother's feelings, providing accurate Burkitt's lymphoma-related information that addresses the health needs and problems of their children with Burkitt lymphoma.