Aim: To enhance nurses' performance regarding care of Newborns in NICUs. Design: A descriptive design was used. Settings: The study was conducted at NICUs in both Maternity and gynecology and Pediatric Hospital that affiliated to Ain shams university hospitals. Sample: A convenient sample of 70 nurses, from the previously mentioned settings during the period of 1 year. Tools: Two tools were used to collect data namely: A structured interviewing questionnaire and standardized observational checklists to assess nurses' knowledge and practice regarding care of newborns at NICUs. Results: The study findings revealed that the competent core are implemented for newborns estimated at around (47.1%) while incompetent (52.9%) of studied sample knowledge regarding care of newborns and that the competent core are implemented for newborns estimated at around (62.9%) while incompetent (37.1%) of studied sample practice regarding care of newborns. there was a highly statistically significant relation between total studied sample knowledge regarding care of newborns at NICUs and their age and attending training courses at level . Also, there was a statistically significant relation between total nurses' knowledge regarding care of newborns at NICUs and their experience While there was a statistically insignificant relation between total nurses' knowledge and their gender, qualification and social condition  and shows that there was statistically insignificant relations between total nurses' practices and their characteristics. Conclusion: The study concluded that the competent core are implemented for newborns estimated at around (47.1%) while incompetent (52.9%) of studied sample knowledge regarding care of newborns and that the competent core are implemented for newborns estimated at around (62.9%) while incompetent (37.1%) of studied sample practice regarding care of newborns. Recommendations: The study recommends continuous assessment and upgrading of nurses' knowledge and practices regarding care of newborns at NICU and Conduct educational programs to improve nurses' performance at NICU based on their actual need assessment.