Background: Nursing education has a major impact on the Sustainable Development Goals. In addition to outlining the variables influencing health, the Sustainable Development Goals offer nurses treatment recommendations. The study aimed to determine the effect of educational guidelines on medical-surgical nursing Students' knowledge and practices regarding sustainable development goals. Research design: A Quasi-experimental research design was utilized to achieve the aim of the study. Setting: The study was conducted at the nursing faculty at Sohag University, Egypt Subjects: A convenient Sample that consisted of the 1st academic years (no. =700). Tools for data collection: A knowledge and practice of Sustainable Development Goals questionnaire. Results: The study's findings demonstrated that medical surgical nursing students had good knowledge and practices related to the Sustainable Development Goals post implementation of the educational guidelines than pre-educational guidelines implementation. Conclusion: The implementation of academic guidelines has a significant effect on improving nursing students' knowledge as well as practice regarding sustainability development goals. Recommendations: The sustainability development education program periodically should be implemented, refreshed, and implemented and its objectives frequently, and the principles of sustainability development to nursing students' curricula.