Abstract: The implementation of the strategies and tools to enhancement performance and patient safety approach in the delivery unit has proven to be crucial in enhancing maternity nurses' knowledge and performance, and reducing nursing errors, increasing team awareness, clarifying team roles, resolving conflicts, improving information sharing, enhancing the quality of care, and ensuring mothers safety in maternity settings. Aim: This study aims to assess the effect of strategies and tools to enhancement performance and patient safety on maternity nurses' knowledge and performance related to teamwork. Settings: The study was conducted in selected maternity units, including word units, the operating rooms, and laboring units, at a teaching hospital and a university hospital in Shebin El-Kom, Menoufia, Egypt. Design: A quasi-experimental research design was utilized. Sample:  A convenient sampling technique was used to recruit 80 maternity nurses from the study settings. Tools: Two tools were used for data collection: the Team STEPPS Knowledge Questionnaire and the Team Performance Observation checklist tool. Results: Post-intervention, the study group showed a significant improvement in knowledge of the Team STEPPS approach (95% vs. 0.0% respectively) and in performance (85% vs. 0% respectively) compared pre intervention. Conclusion: implementation of the Team STEPPS significantly enhanced maternity nurses' knowledge about teamwork. Moreover, the maternity nurses who received Team STEPPS training would have a higher level of performance toward Teamwork STEPPS. Recommendations: The study recommends the reinforcement of the Team STEPPS strategies training to include all maternity units within the healthcare facility.