Background: Newborns are at a much-increased risk of neonatal jaundice due to the relative adverse effects of neonatal hyperbilirubinemia. Aim: This study aimed to evaluate the effect of awareness program on newly mothers' knowledge and practices regarding neonatal jaundice. Research design:  A quasi-experimental research design (pre-and post-test) was used to achieve the aim of this study.  Setting:   The study was carried out in the pediatric outpatient clinics at Mansoura University Hospital. Sample: A convenient sample of 100 newly mothers was included in the previously mentioned setting their ages between 18 and 35 and accepted to participate in the study sample. Tools: Tool (I):  Newly Mothers' Personal Data such as age, level of education, occupation, and residence, Tool (II): Newly Mothers' Knowledge Assessment Sheet regarding to Neonatal Jaundice, and Tool (III): Newly Mothers' Practices Assessment Sheet regarding to neonatal jaundice. Results: The study findings revealed that mothers had a higher score of knowledge and reported practice post-implementation of the awareness program compared to pre-implementation regarding neonatal jaundice. There was a positive correlation between mothers' total knowledge and practice scores at the p= 0.001 level of significance regarding neonatal jaundice post-implementation. Conclusion: Implementing of awareness program has a positive effect on newly mothers' knowledge and practices regarding neonatal jaundice. Recommendations: An awareness program is recommended for newly mothers to improve their knowledge and practices regarding neonatal jaundice