Background:  Improving the professional identity of nursing intern is significant for enhancing the number of new registered nurses and easing the shortage of nursing personnel. The clinical internship is a key period for the formulation of professional identity. Aim: the current study aimed to assess factors affecting professional identity among nurse interns'. Design: A descriptive cross –sectional design was used to carry out this study. Setting: the study was conducted at Ain Shams University Hospital where nurse interns are having their training internship program, Subjects: The subjects of this study was include (120) nurse interns selected by simple random sample technique. Tools of Data collection: the current study was collected by professional identification scale. Results: More than three quarters (78.3%) of nurse intern had a high level of professional identity . While less than one quarter (21.7%)of them had low level of professional identity. As for total nurse interns' views of the factor affecting on professional identity, points to statistically significant relations with their, and. Moreover, a significant association is observed between place of birth, graduation grade, first choice is nursing profession and professional identity Conclusion: the current study findings, concluded that the majority of nurse interns' had high level of professional Identity. Recommendations: the study recommended that the nursing colleges and clinical nursing departments collaborate to create a tough and broad career planning curriculum for nursing interns, aims to enhancing their professional identity.