Background: Enhancing staff nurses' perception toward artificial intelligence is essential and attracts a role in the future nursing generation. Aim of the study: to investigate artificial intelligence is threatening the existence of nursing and nurses' perceptions and attitudes. An expolatory research design was used. Sample: a convenient sample (All available) of staff nurses working in the units with a total number of 455 staff nurses during data collection periods.Setting: This study was conducted in Minia University Hospital, a pediatric, obstetric university hospital, and a kidney disease and urology hospital. Data collection tools: Perceptions of staff nurses regardingthe artificial intelligence questionnaire and general attitudes towards the artificial intelligence questionnairewere used. Results: The nurses had a moderately positive perception and attitude toward artificial intelligence, with the highest perception related to artificial intelligence ability to reduce medical errors (3.80 ± 1.202). No statistically significant differences were found between the different hospitals (Pediatric , obestatric, kidney and urology hospitals), ages, gender, and residence of the studied nurses, and mean scores of perception and attitude toward AI in healthcare. Conclusion:Artificial intelligence improves nursing, not threatens it. Nursing professionals percevie AI can improve patient care, efficiency, and clinical decision-making. Also, job displacement concerns, comprehensive training, benefits and concerns, improved patient outcomes, collaboration, ethics, and patient-nurse relationships, nursing professionals believe artificial intelligence can improve care. Recommendation:Provide comprehensive training, consider both benefits and concerns, improve patient outcomes, foster collaboration, address ethical considerations, and maintain patient-nurse relationships to maximize the benefits of AI in healthcare.