Background: Sacrococcygeal pilonidal sinus disease (SPSD) patients should have the following treatment: By increasing granulation and fibrosis, phenol treatment speeds up healing because of its mild sclerosing actions and simplicity of administration. An efficient method for health care education is simulation training, directed by Kern's six-step curriculum-building process. It increases decision-making abilities, lowers the chance of errors, and strengthens clinical practice. Hence, the current study aimed to evaluate the effect of a nursing orientation training program based on Kern's Six-Step approach on nurses' performance regarding Phenolization dressing application. Design: Quasi-experimental research design was used to achieve the aim of this study. Setting: The current study was applied in the General Surgery Department at Sohag University Hospital. Subjects: Fifty nurses employed in the preselected settings were included in the study using a convenient sample procedure. Three tools were used for data collection; Tool (1): Nurses' data; Tool (2): Nurses' knowledge regarding Phenolization dressing technique (pre/post); Tool (3): Nurses' practice regarding phenolization dressing technique (pre/post). Results: The findings of the study demonstrated a highly statistically significant difference in nurses' knowledge and practices about the penalization dressing technique between the pre-and post-Kern Six-Step training programs. Furthermore, the study nurses' total knowledge and total practice scores regarding the Phenolization dressing technique showed a statistically significant positive correlation. Conclusion: Using Kern's Six-Step Approach to design a nursing orientation training program had a positive effect on the enhancement of surgical nurses' knowledge and practice regarding phenolization dressing techniques. Recommendations: Nurses working in surgical facilities should get a nursing orientation training program that adheres to Kern's Six-Step method, emphasizing the significance of the phenolization dressing technique.