Background: Vasectomy is a safe and permanent method of contraception. However, it is not widely used in developing countries. Aim of the study: To assess the effect of vasectomy counselling program on women‘s knowledge, attitude and acceptance. Design: A quasi – experimental one group pretest- post-test design was utilized. Setting: The study was carried out at family planning clinic in the obstetrics and gynaecology department, Sohag University /Egypt. Sample: A convenient sample of 100 women was involved. Data collection tools: Three tools were used to collect data: Tool (I) Women's personal data questionnaire, Tool (II) Women's knowledge questionnaire regarding vasectomy, Tool (III) Women's attitude and acceptance scale. Results: There was a highly statistical significant difference between pre and post total knowledge , attitude and acceptance scores of women to use vasectomy with  P <0.001. Conclusion: The counselling programme had a significant positive effect on women's knowledge, attitude, and acceptance, as evidenced by an increase in total mean scores in post-tests compared to pre-test mean scores. Recommendations: Community awareness campaigns  via all types of multimedia to provide accurate information about the vasectomy is highly recommended to increase awareness and dispel myths about vasectomy  &raise vasectomy acceptance.