Background: Helicobacter pylori infection invariably causes active chronic gastritis. Aim of the study:  to assess mothers' knowledge, practice, and home-based management regarding the prevention of helicobacter pylori in Barout, Beni-Suef. Research design: A descriptive design was utilized to achieve the aim of the current study. Sample: A convenient sample composed of all mothers attending the Mother and Child Health Center in Barout Village in Beni-Suef Governorate. Tool of data collection: Tool (I) Part (I): Mothers' demographic characteristics, Part (II) Knowledge questionnaire for mothers Part (III):  mothers reported practices regarding the prevention of H. pylori. Results: The findings of this study revealed that 37.7%  of studied mothers had poor knowledge levels and 80.3% of them had unsatisfactory home-based practices regarding H. Pylori Infection. Also a statistically significant positive correlation between total knowledge and home-based practice of the studied mothers. Conclusion: Mothers' knowledge, and home-based management practices regarding the prevention of helicobacter pylori in Barout village at Beni-Suef governorate need to improve. Recommendation: Regular training programs to attend women in Mother and Child Health Center to improve their knowledge and home-based management practices regarding the prevention of Helicobacter Pylori which can reduce the morbidity of the disease.