Background: Violence against women is widespread, according to estimates from the World Health Organisation (WHO), more than one-third of women worldwide have been victims of either physical or sexual violence. Aim: to evaluate effect of Nurse Led Training Program on nurses' knowledge regarding violence against women and safe women's services. Design: Quasi experimental research design was utilized in this study. Setting: It was conducted at Qolta MCH, Old El-Eman Hospital& El-Arbeheen Hopital at Assuit city. Sample: purposive sample of all nurses working in the previous mentioned settings (144). Tool: Structured interviewing questionnaire. Results: There is highly statistical significance improvement on total knowledge level about violence against women and safe women's services following implementation of training program. Conclusion: Implementation of the Nurse Led Training Program was effective in improving nurses' knowledge regarding violence against women and safe women's services. Recommendations: Implementing this Nurse Led Training Program with A booklet for nurses on the other health centers to improve & update their level of knowledge regarding violence against women and available safe women's services.