Background: Percutaneous nephrostomy tube (PCN) is a widely utilized interventional procedure for upper urinary diversion and decompression of the renal collecting system. Caring for patients with a percutaneous nephrostomy is a critical component of nursing management to minimize complications and improve the health status of those patients. However, there is inadequate information for nurses as well as a lack of evidence and guidance (Buachuen S. (2022). The study aimed to investigate the effect of the educational guidelines on nurses' performance regarding percutaneous nephrostomy tube. Study design: A quasi-experimental design was used to conduct this study (pre/post-test). Setting: The research was carried out in the urology department at Suez Canal University Hospital. Sample: A convenient sample of 40 of all available staff nurses working in the urology department. Tools of data collection: One tool was used for data collection. Tool I: "Nurses' Performance Assessment Sheet" which was composed of three parts; 1. Demographic data for nurses, 2. Nurse`s knowledge questionnaire and 3. Nurses practice observational checklist. Results: The study result revealed that there was a highly statistically significant positive correlation between total knowledge scores and total practice scores of the studied nurses' pre and posts educational guidelines implementation regarding percutaneous nephrostomy tube. Conclusion: It was concluded that the educational guidelines had a positive effect on improving nurses' knowledge and practice regarding percutaneous nephrostomy tube. Recommendations: Ongoing educational programs and in-service training programs about the percutaneous nephrostomy tube should be provided to nurses to upgrade their knowledge and skills.