Background: Considering the COVID-19 emergency, distant learning was selected as the preferred mode of teaching. Practically everywhere on the globe has attempted it. Using internet-based digital resources to enhance learning is the process of distance learning. Aim of the study: Was to assess the effect of Distance Learning on Mothers' Quality of Life during COVID-19 Pandemic. Setting: The study was conducted at 50% of Governmental Benha Preparatory Schools. The total number of preparatory schools in Benha city is about 16 schools, 8 schools were selected to be included in the study and were chosen by simple random technique. Sample: The snowball sampling technique was used and included 270 mothers. Tools: Two tools were utilized. Tool I: Pre-designed questionnaire to assess the socio-demographic characteristics, family characteristics of the mothers and mothers' knowledge regarding distance learning. Tool II: The World Health Organization Quality of Life brief version questionnaire. Results: 48.5% of studied mothers aged from 35 to less than 45 years with mean age 34.68±7.65years, 48.5% of them had university education. 39.6% of them had poor total knowledge regarding distance learning.60.7% of studied mothers had low total quality of life and 22.3% of them had moderate total quality of life and only 17% of them had high total quality of life regarding to distance learning. Conclusion: A highly statistically significant relations between total quality of life and socio-demographic characteristics of studied mothers regarding age, educational level, residence, occupation, and marital status (p< 0.001). A highly statistically significant relations between total quality of life score regarding distance learning and family characteristics of mothers as number of children, children educational level and times of electronic learning classes (p<0.001). Furthermore, there were statistically positive correlation between studied mothers' total level of knowledge and their total quality of life score (p<0.001). Recommendation: Creating counseling programs in facing these crises for families, training parents on modern education strategies, designing guidance programs and working on them for all groups of society.