Egg freezing has become a widespread phenomenon over the past ten years, and with the increase in the age of marriage and the lack of opportunities available to women, developments in egg-freezing technology have given women an opportunity to control their reproductive prospects. This study aims to evaluate the effect of an instructional program on female students' knowledge, attitude, and challenges. towards egg-freezing. Design: A quasi-experimental research design was utilized in this study.Setting: The study was conducted in the Faculty of Nursing at Kafr El-Sheik University. Egypt. Sample: A convenience sampling approach chose 200 female students. These female faculty students are in 1^st, 2^nd, 3^rd, and 4^th  grades. Tools: a structured interview questionnaire involved three tools; demographic data, Egg-freezing knowledge, attitude assessment tools, and challenges assessment tools. Result: The findings demonstrated significant improvements in the overall knowledge and attitude levels of female students regarding egg freezing after the instructional program, as compared to their pre-program levels. This improvement was observed in all knowledge and attitude items, with a statistical significance of p≤0.001. Additionally, there was a notable reduction in the challenges faced by female students concerning egg freezing following the implementation of the instructional program. This reduction was highly statistically significant, with a difference observed at p<0.001. Conclusion: This study concluded that the female students' knowledge, attitude, and challenges will be improved after implementing the instructional program regarding egg freezing. Recommendation: Continuing health educational programs to improve knowledge and attitude regarding egg freezing and other research on the problems that women face during egg freezing.