Background: Resonant leadership provides the nurse managers with the opportunity to design and manage the environment. Objective: The current study aimed to evaluate the influence of program regarding resonant leadership on knowledge and practices of nurse managers. Subjects and methods: Setting: The study was conducted at Emergency Hospital and Tanta University Main hospital. Subjects:  Nurse Managers and charge nurses (n = 66) at Tanta Main University and Emergency Hospitals, also 322 of staff nurses. Tool: Three tools were used; (1) Nurse Managers' resonant leadership knowledge structured questionnaire; (2) Nurse Managers' resonant leadership practice self-report; and (3) Nurses' perception about nurse managers' resonant leadership structured questionnaire. Results: The majority (87.9%) of nurse managers had low level of knowledge regarding resonant leadership and none of them had satisfactory level of resonant leadership practice pre-program.  While all of them had high level of knowledge and practice at immediate, which slightly decreased to the majority (86.4% and 83.3%) of them after 3 months of program respectively. None of nurses described that their nurse managers practiced high resonant leadership. While all (100%) of them reported that their nurse managers had high level at immediate, which slightly decreased to 84.5% of them after 3 months of program. Conclusion: Nurse Managers at Main Tanta University Hospital and Emergency Hospital showed low level of knowledge and practice regarding resonant leadership. While they develop their resonant leadership skills after implementation of needed programs. It is recommended to organize frequent periodic advancement program for nurse managers about resonant leadership.