Background: In the latter stages of many illnesses, such as diabetes mellitus, limb amputation is a life-altering treatment that has social, psychological, and functional repercussions that lower the patient's quality of life. Objective: The current study aimed to assess the health-related quality of life for patients with diabetic foot amputation. Patients and methods: A descriptive exploratory study was conducted at the Outpatient Clinic affiliated to Beni-Suef University Hospital. A total of 70 diabetic patients, 3 to 18 after amputation operation for follow up, were recruited using a purposive sample. Data of participants were collected using two tools; (I): Structured interviewing questionnaire, it included three parts; socio-demographic characteristics of patients,medical history of patients and knowledge assessment questionnaire. (II)World Health Organization quality of life SF36 questionnaire. Results: About51.4% of studied patients were in the age group 50 - <60 years, 58.6% of them had unsatisfactory total knowledge level regarding diabetic foot and amputation, and 60% of them had unhealthy or poor quality of life. There was no statistically significant correlation between total knowledge and total quality of life regarding diabetic foot amputation. Conclusion: Developing and implementing an educational programme for patients with diabetic foot amputation for improvement of their quality of life is strongly and urgently needed.