Two hundred samples from 5) whole broiler chickens carcasses (30 frozen and 20 fresh)
represented by breast, thigh, liver and kidney samples (50 of each) were collected from Minuofia
governorate for detection their residual level of oxytetracycline (OTC) and sulfamethazine (SMZ). The
detection and estimation of such antimicrobial were applied by FPT and HPLC. Then positive
 samples for detection of such antimicrobial residues. The result showed that in total the 
positive  were o 14%, 20%, 28% and 36% total the positive samples of PPT were LA/o, 
20% and 36% for OFC residues while they were 10%, 12%, 16% and
10% for SVEZ residues for each sample of breast, thigh, liver and kidney respectively. The average
concentration of OTC by HPLC in positive frozen samples by (mg/kg) were (0.198 + 0.032), (0348+
0.0617). (1.423 + 0.303) and (1.952 + 0.316) while in SMZ were (0.2625 + 0.060), (0.4274 0.097)
(0.686#0.1225) and (1.410 + 0.275) for breast, thigh, fiver and kidney respectively. Accurately, 3.33%,
13,33, 16.67°% and 26.67% of the examined samples for OTC and 6.67%, 10%, 13.33% and 16.67% of
the examined samples for SMZ were unaccepted concerning breast, thigh, liver and kidney respectively.
The average concentration of OTC by HI LC in positive fresh samples by mg/kg were 0.417 + 0.087, 0.665
+ 0.231, 2.07 # 0.343 and 2.645 + 0.597 while of SM L they were 0.370 + 0,090, 0. 5234 + 0.15, 1.268 + 0.226
and 2.0525 + 0.333 concerning breast, thigh, liver and kidney respectively. Accurately, I5'%, 20% and
40% and 35% of the examined samples for OTC and 15%, 15%, 20% and 25% of the examined samples
for SMZ were unaccepted for breast, thigh, liver and kidney respectively. Cooking process have reduced
effect on SMZ and OTC .but in OTC give degradation product have more toxic effect than the parent
drug. The most target organ was liver in incidence of SMZ and OTC and their concentrations followed
by thigh and then breast.