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355071

Effect of conjugated linoleic acid isomer -cis-9, trans-11- on the cytological parameters of post-thaw ram spermatozoa

Article

Last updated: 03 Jan 2025

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Subjects

-

Tags

Theriogenology / reproductive technology

Abstract

The present study aimed to evaluate the effect of addition of conjugated linoleic acid isomer-cis-9,trans-11- (CLA) to egg yolk-Tris glycerol diluent on the cytological parameters of post-thaw ram spermatozoa. Different concentrations of CLA (50, 100 and 150 μM) were added to the cryopreservation medium of ram semen. Five fat-tailed rams were used and their ejaculates were pooled and processed. Conjugated linoleic acid, which had oily presentation, were prepared by dissolving it in ethanol. Treated diluted semen was cooled, equilibrated and subjected to cryopreservation. The motility characteristics of the post-thaw spermatozoa were analyzed objectively; plasma membrane integrity by hypo osmotic swelling test (HOST), alive and abnormal sperm percentage by eosin negrosin stain and hanocock's method and acrosomal integrity by spermac stain. Results showed that CLA (100 and 150 μM) increased significantly (P< 0.05) the live sperm percentage, HOST and intact acrosome percentage of frozen thawed ram semen compared to control. Regarding the effect of CLA on the total sperm abnormalities, the 150 pM CLA was the only concentration that decreased significantly (P <0.05) the percentage of abnormal sperm cell compared to control (15.0 vs 19.4%). However, concentrations (50, 100 and 150 1M) tested decreased significantly (P <0.05) the percentage of abnormal sperm (21.6%, 17% and 15%, respectively compared to control with ethanol 29.2%). The effect of different concentrations (50, 100 and 150 μM) of CLA on the progressive motility percentage was non significant. It could be concluded that, CLA improved the freezability of ram spermatozoa in terms of membrane and acrosome integrity and decreased sperm cell abnormalities.

DOI

10.21608/vmjg.2024.355071

Keywords

Semen cryopreservation, conjugated linoleic acid, ram and fatty acids

Authors

First Name

H.

Last Name

Elsherbiny

MiddleName

-

Affiliation

Department of Theriogenology Faculty of Veterinary Medicine, Cairo University; Giza, Egypt

Email

-

City

-

Orcid

-

First Name

M.

Last Name

Abou-Ahmed

MiddleName

-

Affiliation

Department of Theriogenology Faculty of Veterinary Medicine, Cairo University; Giza, Egypt

Email

-

City

-

Orcid

-

First Name

A.

Last Name

Ghallab

MiddleName

-

Affiliation

Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University; Giza, Egypt

Email

-

City

-

Orcid

-

Volume

63

Article Issue

2

Related Issue

44993

Issue Date

2017-04-01

Receive Date

2024-05-19

Publish Date

2024-05-19

Page Start

15

Page End

20

Print ISSN

1110-1423

Online ISSN

2537-1045

Article File

VMJG_Volume 63_Issue 2_Pages 15-20.pdf

PDF . 3.3MB

Link

https://vmjg.journals.ekb.eg/article_355071.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=355071

Order

355,071

Type

Original Article

Type Code

544

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

Effect of conjugated linoleic acid isomer -cis-9, trans-11- on the cytological parameters of post-thaw ram spermatozoa

Details

Type

Article

Created At

23 Dec 2024