The recalcitrant nature of polyvinyl chloride causes significant environmental challenges during production and disposal. This study aimed to assess the ability of fungi isolated from the washing tank in a plastic production factory to biodegrade polyvinyl chloride (PVC). Over 60 days, fungi isolated were incubated with plastic pieces in Bushnell Haas medium. These strains were identified as Coriolopsis gallica (F1), Aspergillus niger (F2)and Aspergillus flavus (F3). After incubation, three methods were selected: Fourier transform infrared (FTIR) analysis, gas chromatography-mass spectrometry (GC-MS), and weight loss experiments, to determine the biodegradation of PVC. The FTIR analysis demonstrated changing peaks, disappearance, and formation of new bonds of the PVC treated compared with the control. A GC-MS analysis revealed the formation of carboxylic acid, alcohol, nitrile and a new compounds during the decomposition of PVC. The results of the weight loss experiment for microbial strains F1, F2, F3 and fungi consortium (FC) were 19, 25.3, 23.6, and 52.6%, respectively. The FC was prepared by combining all three fungi isolates. The present study concluded that these isolated fungal strains have potential for partial biodegradation of PVC plastics. Nevertheless, the results suggest that fungi consortium plays a significant role in the degradation of PVC in aqueous environment.