Background: Accidents are the leading cause of death among children and can lead to significant disability if not promptly and appropriately managed. Mothers, as caregivers, play a vital role in providing immediate assistance to their children during such incidents. First aid intervention can lower the disability and increase the chances of survival for the injured child. Aim: This study aimed to assess mothers' knowledge and practices about accident first aid for their children. Research design: A descriptive design was utilized for conducting the study. Setting: This study was conducted in the pediatric outpatient clinics at Beni-Suef University Hospital. Subjects: A convenience sample of 340 mothers accompanying their children to the previously mentioned settings. Tools for Data Collection: Two tools were used: Tool (1) a structured interview questionnaire format, and tool (2) a mother's reported practices. Result: The study revealed that 47.35% of the studied mothers were in the age group of 30 to less than 40 years and 60.88% of them lived in rural areas. 32.05% of mothers graduated from secondary education. Also,80% of them had unsatisfactory level of knowledge regarding accident first aid for their children. Moreover, 93% of studied mothers had incomplete level of reported practices regarding first aid. Conclusion: The majority of studied mothers had unsatisfactory level of knowledge regarding accidents, first aid for their children, and incomplete level of reported practices. Recommendations: Training courses about first aid must be offered to mothers in pediatric settings. Health providers should continue their essential information to mothers and training courses about first aid periodically in the pediatric settings where they are working. TV and other media must also offer information about common children's accidents, and the first aid needed for each type.