A total of 90 randomly collected milk samples from different localities in Ismailia Governorate for determination of some (antibiotic residues, heavy metals and chemical preservatives). Results revealed that qualitative detection of β-lactams and fluoroquinolones residues in milk samples by using Airflow™ Strip Test Kit were 42.2% and 21.1%, respectively. Quantitative
detection by HPLC analysis revealed that 32.2%, 7.8 % and 21.1 % of examined milk samples were contaminated with Amoxicillin, Ceftrixone and ciprofloxacin, respectively with the mean concentrations of 6.95± 1.36 ,8.67±2.42 and 8.68 ±1.9 ppb, respectively. The antibiotic residues after boiling was significant decreased to 1.04 ppb, 1.09 ppb and 6.33 ppb for amoxicillin, ceftriaxone and ciprofloxacin residues, respectively with the reduction percent of 85%, 87.4% and 27%, respectively. Heavy metals were detected by using atomic absorption spectrometer. The incidence of lead in examined milk samples were 75.5% with the mean concentration 0.4555± 0.0828ppm. 7.77 % of all examined milk samples shown to be adulterated with formalin. These contaminants constitute a public health hazards to many consumers and attention should be pay to prevent reaching of such contaminant to consumers