Background: Creation of biocompatible compounds using nanotechnology as anticancer agent, on other hand, were highly effective against MCF-7 breast cancer cells and had reasons of cell death, making it one of the novel approaches in field of cancer therapy. Aim: Impact of Ag nanoparticles using Musa acuminata on pathogenic bacteria. Methods: 125 samples were collected with various infections. All necessary examinations for preliminary diagnosing of isolated bacteria were carried out and confirmatory diagnosis of these isolates by the Vitek- 2 system. Biosynthesis of silver nanoparticles was conducted via green methods by using Musa acuminata alcoholic extracts, and properties of silver nanoparticles were defined by spectrophotometer. Results: The largest Ag NPs  75 nm for alcoholic Musa acuminata extract and FTIR analysis showed presence of flavonoids and tannins in abundance in Musa acuminata fruits, using an agar diffusion assay, silver nanoparticles biosynthesized from Musa acuminata isolates demonstrated antimicrobial efficacy against E. coli, S. epidermidis, K. pneumoniae, S. aureus and P. aeruginosa. At 12,5 mg/ml Ag NPs, it was shown that growth of these bacteria was suppressed. Concentrations of nanoparticles (12,5, 25, 50, 100 and 200 mg/ml) exhibited greatest inhibitory effects on S. aureus (20 mm), E. coli (18 mm), K. pneumonia (20 mm), and P. aeruginosa (15 mm) and S. epidermidis (24 mm). Conclusion: Klebsiella pneumonia was the most common infection in diabetic foot. Ag NPs could be used as potent nanomedicines that are ideal substitutes for crude extract in treatment of diabetes mellitus.