Introduction: The common type of cancer among women is epithelial ovarian carcinoma (EOC); this is because there aren't sufficient early diagnostic indicators. Each tumor marker has a special set of use for screening, diagnosing, evaluating response to therapy, and monitoring for cancer recurrence. Objective: The aim of the current is to assess role of biochemical markers in diagnosis of ovarian cancer patients, such as serum total sialic acid, Glycoprotein, Ferritin, and Cancer Antigen 125. Additionally, the impacts of oxidative stress in cancer cells that initiate apoptotic pathways will be investigated. Patients and methods: The participants of this study comprised 36 women with epithelial ovarian cancer patients, and 36 women with benign ovarian mass (pathological controls). Other 36 women were considered normal healthy controls, who attended for treatment in Al-Amal National Cancer Hospital from January 2022 till June 2022. Results: Epithelial ovarian cancer patients had a mean age of 52.8 (SD 9.7) years, and benign ovarian mass (pathological controls) had a mean age of 49.6 (SD 5.9) years. Normal healthy controls had mean age of 50.7 (SD 3.8) years. Serum TSA, glycoprotein, ferritin, and CA-125, can provides useful information for patients in a diagnosis of ovarian cancer particularly in early-stage disease. Ferritin discriminates between healthy controls and patients with epithelial ovary cancer's, particularly in early-stage disease. In order to test for EOC, serum ferritin and CA125 together offer highest level of diagnostic efficiency. Conclusion: Early identification and diagnosis of metastasis in women with ovarian cancer is beneficial for therapy and can be utilized to determine why cancer cells develop chemoresistance.