Background: Hepatocellular function is compromised in liver cirrhosis patients, and albumin production is decreased. Objective: The aim of the current study was to evaluate clinicians' understanding of the International Guidelines-supported evidence-based indications for using human albumin (HA).  Patients and methods: A survey was conducted on specialized Gastroenterology Physicians at Al-Rajhi Liver Hospital and other Hospitals in Assiut, who regularly treat patients with liver cirrhosis. The study was carried out between October 2020 and December 2021. Physicians' knowledge was assessed using Tool I then re-evaluation was done to determine the effect of designed evidence-based indications (Tool II) for HA use on Physicians. Results: There was statistically significant higher mean knowledge score at the post test compared to baseline (7.6±0.9 compared to 3.6±1.8, respectively). Regarding knowledge score categories, good knowledge of Physicians statistically increased from 52.4% at the baseline to 100% at the post test. There was statistically significant higher mean difference in function of HA knowledge score in academic doctors (3.9±1.7) compared to nonacademic doctors (2.7±1.9). There was statistically significant higher mean difference in function of HA knowledge score in doctors with experiences ≥5 years (4.0±1.6) compared to doctors with experiences <5 years (3.3±1.9). Conclusion: The level of knowledge to function and the International Guidelines of albumin use in patients with liver cirrhosis significantly increased in the post test especially among nonacademic doctors and fresh graduate doctors.