COVID-19 emerged as a global threat, affecting millions of people worldwide, as a result, personal protective equipment is an important part of a country's efficient COVID-19 emergency response. The study aimed to assess personal protective equipment's adverse reactions and psychological preparedness among critical care nurses during COVID-19. A descriptive research design was used to conduct this research. This study was carried out in intensive care isolation units from different hospitals in Sohag city. Convenient nurses in intensive care isolation units constituted the sample. Web-based self-administrated questionnaire was used to collect date. The results of present study showed thatthe majority of the studied nurses were females and aged (20- 30) years. Majority of nurses felt anxious, Chest tightness or suffocations, shortness of breathing, dizziness or palpitations, thirst, and skin dehydration. The current study revealed that nurses in intensive care isolation units are affected with various adverse effects related to PPE and psychologically felt anxious.