Background: An awareness of professional values, cultural differences, and bases help to avoid ineffective communication in stressful situations which can help staff nurses in preventing their feeling of burnout. Aim: To assess nurses' professional values and its relation to their Burnout in Minia university hospitals. Research design: A descriptive cross- sectional research design. Subjects: A representative sample 30% from total staff nurses all staff nurses who working in Minia University Hospitals (N= 337). Setting: The study was conducted in Minia University Hospitals, Egypt. Tools of data collection: included two tools; Nurses Professional Values Scale (NPVS), and Maslach's Burnout Inventory scale. Results: Staff nurses had moderate mean score level for all subscale of professional values; also there were more than three quarter (78.3%) of the staff nurses had moderate score level of total professional value, less than one quarter (20.8%) of them had high score level degree. Also, there were less than two third (62.9%) of staff nurses had high total score levels of Maslach's burnout inventory; more than one third (37.1%) of them have moderate score level; and no one of them had low burnout level score. Conclusion: The findings of this study revealed that staff nurses had moderate to high level of professional values perception as well as their burnout level. Also, there were negative correlation between professional values and level of burnout. Recommendation: Training programs should be designed continuously for staff nurses to improve their professional values perception and decrease their burnout level.